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In the article, Fraxatin, a conserved mitochondrial protein, in the hydrogenosome of Trichomonas vaginalis, the fraxatin protein is studied to examine its phylogenetic history. Fraxatin is involved in cell iron metabolism and is found in animals ranging from eubacteria to mammals. Some methods used included cloning, sequence analysis, Polymerase Chain Reaction (PCR) to examine RNA transcription, and immunoflourescence microscopy.

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