22 This approach yielded a strong nuclear and cytoplasmic

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Replica Prada Top of pageResultsDeficiency in the nuclear expression of HMGB1 in two transplantable tumor modelsHMGB1 expression was determined in paraffin embedded CT26 colon carcinoma and MCA205 sarcoma tumors using immunhistochemical analysis using a specific anti HMGB1 antibody.22 This approach yielded a strong nuclear and cytoplasmic staining in cells that were cultured in vitro, pelleted and paraffin embedded (Figure 1a). Although all CT26 and MCA205 tumor cells stained positively after in vitro culture Prada Outlet, a large fraction of them became negative after in vivo passage in immunocompetent BALB and C57Bl6 mice, respectively (Figure 1a). Surrounding cells of the tumor stroma or lymph node exhibited a nuclear HMGB1 staining (Figure 1a, right panels) as an internal control, pointing to the differences between normal and cancer cells that arise in vivo Replica Prada.

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